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Description
Rat ATG5 (Autophagy Protein 5) ELISA KitThe Rat (ATG5) Autophagy Protein 5 ELISA Kit measures Autophagy protein 5 in Rat samples. The plate has been pre coated with Rat ATG5 antibody. ATG5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ATG5 Antibody is added and binds to ATG5 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ATG5 antibody. After incubation unbound Streptavidin HRP is washed away during a
The Rat (ATG5) Autophagy Protein 5 ELISA Kit measures Autophagy protein 5 in Rat samples. The plate has been pre-coated with Rat ATG5 antibody. ATG5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ATG5 Antibody is added and binds to ATG5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ATG5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat ATG5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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