Human TAP (Trypsinogen Activation Peptide) ELISA Kit

Human TAP (Trypsinogen Activation Peptide) ELISA KitThe Human TAP (Trypsinogen Activation Peptide) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Human TAP. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to Human TAP. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution

The Human TAP (Trypsinogen Activation Peptide) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human TAP. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Human TAP. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TAP in the samples is then determined by comparing the OD of the samples to the standard curve.