SKU: 551459918

Bovine IL-4 (Interleukin 4) ELISA Kit

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Description

Bovine IL-4 (Interleukin 4) ELISA KitThe Bovine IL 4 (Interleukin 4) ELISA Kit measures IL 4 in Bovine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection

The Bovine IL-4 (Interleukin 4) ELISA Kit measures IL-4 in Bovine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-4 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-4 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 551459918

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Amazon Customer
Cuba, US
★★★★★ 5
Funny story for kids.
Format: Paperback
My son who is 7 years old can’t get enough of this book! It a funny story that any child in his age bracket will enjoy. It was a perfect addition to his Easter basket.
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Reviewed in the United States on May 8, 2021
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Kathryn Reynolds
Lake Worth, US
★★★★★ 5
Quality
Format: Hardcover
Grandson loves books
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Reviewed in the United States on May 15, 2026
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Lindsay L Huntington
Charlottesville, US
★★★★★ 5
A book every teacher/parent should read!
Format: Hardcover
I LOVE this book because the "bad seed" reminds me of my son. I don't believe there are any "bad kids" in the world but I'm sure kids look at themselves as bad if that is all they ever hear. I love how this story gives a sympathetic backstory on how he got "so bad" and how he is trying to do better and change his bad ways. Even though my son isn't perfect, he sure does try to do good and his behavior improves when people notice the POSITIVE instead of the negative. I absolutely love the last lines of the book where the other seeds are gossiping (another lesson) saying, "Hey! There goes that bad seed," and another seed sticks up for him and says, "Actually, he isn't that bad anymore." Then the "bad seed" turns around with a smile on his face and says, "I heard that." Moral of the story.....children are always listening. Focus on the positive rather than the negative and a child's self-talk and behavior will drastically improve. My son's 2nd grade year was so negative and he was always getting into trouble. I rarely heard the good and neither did my son. His 3rd grade year was amazing. The teacher really focuses on pointing out his good choices and what a difference that made in my son's attitude toward school. Thank you Jory John for writing this book. I also love the book, "I Love You Already" which teaches readers about friendship, love, patience, loyalty and forgiveness. :)
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Reviewed in the United States on October 2, 2018
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Patty Rosebourgh
Bozeman, US
★★★★★ 5
Kids learn to keep going
Format: Hardcover
Used for read aloud
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Reviewed in the United States on February 18, 2026
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Jamie
Whiting, US
★★★★★ 5
The Bad Seed is not so bad after all!
Format: Hardcover
Great little book!
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Reviewed in the United States on December 21, 2025

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