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Description
Protein A magnetic microspheresProduct Name Protein A magnetic microspheres Product Overview Protein A magnetic beads are a special biotechnology product commonly used in immunoprecipitation (IP) experiments in biomedical research. They are made of superparamagnetic material coated with Protein A, a protein isolated from Staphylococcus aureus that specifically binds the Fc fragment of several mammalian IgGs. Technical Parameter Concentration: 30 mg mL Solvent: water Diameter: ~1um
Product Name
Protein A magnetic microspheres
Product Overview
Protein A magnetic beads are a special biotechnology product commonly used in immunoprecipitation (IP) experiments in biomedical research. They are made of superparamagnetic material coated with Protein A, a protein isolated from Staphylococcus aureus that specifically binds the Fc fragment of several mammalian IgGs.
Technical Parameter
Concentration: 30 mg/mL
Solvent: water
Diameter: ~1um
SPA load : 70-75ug/mg
Surface potential:~ 29 mV
Magnetic content: 35%-45%
Product Features
High affinity: Protein A magnetic beads are able to bind specifically to Fc fragments of a wide range of mammalian IgGs with high affinity.
Superparamagnetic: These magnetic beads are made of superparamagnetic material, which can be rapidly magnetised and show magnetic properties under an applied magnetic field, facilitating experimental manipulation and separation.
Low non-specific binding: Protein A magnetic beads are usually pre-closed to reduce non-specific binding and improve the specificity and reproducibility of experiments.
High capacity: Protein A magnetic beads have a high binding capacity and can capture a large number of antibodies or antigens.
Application Fields
Application Fields
Immunoprecipitation assays: In cell lysates, the specific binding ability of Protein A magnetic beads allows for the capture of protein complexes bound to their antibodies for downstream analyses such as protein blotting (Western Blot) or mass spectrometry.
Antibody purification: Due to the high affinity of Protein A for IgG, Protein A magnetic beads can be used to purify specific types of antibodies from mixed samples.
Affinity Chromatography: In affinity chromatography, Protein A magnetic beads can be used as a solid phase carrier for the separation and purification of target proteins.
Related Information
Sealed, avoid light, and keep at 4¡ãC Expiry date: Six months before unsealing.
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