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Description
Mouse HIF-1A (Hypoxia-inducible Factor 1Α) ELISA KitThe Mouse (HIF 1A) Hypoxia inducible Factor 1 ELISA Kit measures Hypoxia Inducible Factor 1 alpha in Mouse samples. The plate has been pre coated with Mouse HIF1A antibody. HIF1A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse HIF1A Antibody is added and binds to HIF1A in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HIF1A antibody. After incubation unbound Streptavidin
The Mouse (HIF-1A) Hypoxia-inducible Factor 1Α ELISA Kit measures Hypoxia-Inducible Factor 1 alpha in Mouse samples. The plate has been pre-coated with Mouse HIF1A antibody. HIF1A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse HIF1A Antibody is added and binds to HIF1A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HIF1A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse HIF1A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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