SKU: 95311452600

Rat IL-18 (Interleukin-18) ELISA Kit

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Description

Rat IL-18 (Interleukin-18) ELISA KitThe Rat IL 18 (Interleukin 18) ELISA Kit measures IL 18 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 18 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.

The Rat IL-18 (Interleukin-18) ELISA Kit measures IL-18 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-18 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-18 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-18 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 95311452600

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sophie
West Palm Beach, US
★★★★★ 5
amazing cuddle toy
Color: Elwin
my dog’s fav toy at the moment. definitely not for hard chewers since it’s not durable. my girl loves carrying it around, squeaking it and cuddling with it. definitely worth the money, super cute and love how much my dog carries it around!
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Reviewed in the United States on June 3, 2026
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Happy Gardener
Whiting, US
★★★★★ 4
Soft non-squeaker
Color: Enno
Our son's dog loved this non-squeaker! Very easy to carry around the house and cuddle with on his bed.
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Reviewed in the United States on November 22, 2025
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WoostaDon
Phoenix, US
★★★★★ 5
Molly loves this
Color: Elroy
My small dog loves this
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Reviewed in the United States on March 4, 2026
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Charlii
Omaha, US
★★★★★ 1
Very disappointed
Color: Enno
The toy was split into pieces in the first 5 minutes of the dog playing with it. Apparently not as durable as advertised.
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Reviewed in the United States on April 16, 2026
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Amazon Customer
Dallas, US
★★★★★ 3
Dog toys need to be sturdy - maybe you should sew them with a 5-thread machine like a serger?
Color: Elroy
My dog's full size teddy is disintegrating, so I ordered this one. It's very cute, but the seams are really weak. I've already repaired 3 seams in just a few weeks.
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Reviewed in the United States on March 13, 2026

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