SKU: 19457208937

Human IL-4 High Sensitivity ELISA Kit

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Description

Human IL-4 High Sensitivity ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti human IL 4 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL 4 existing in the

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti-human IL-4 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-4 existing in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-4 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, IL-4 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.03pg/ml

Detection Range: 0.25-16 pg/ml

Recovery Range: 94-105%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 4 (IL-4) is a monomeric cytokine of about 18-20kda that can induce naive helper T cells (Th0 cells)Differentiation into Th2 cells. After being activated by IL-4, Th2 cells then secrete IL-4 in a positive feedback manner. The cell type that secretes IL-4 to induce Th0 cell differentiation has not yet been identified, but recent studies have shown that basophils may be this effector cell. IL-4 is closely related to IL-13 and has similar functions.

IL-4 can also promote rhabdomyosarcoma to undergo mitosis, dedifferentiation and metastasis. It can be observed that IL-4 and other Th2 cytokines are involved in airway inflammation in allergic asthma patients.

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SKU: 19457208937

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Monique Rodriguez
Phoenix, US
★★★★★ 5
Great for sublimation
Color: 1BK/1C/1Y/1M
Great for sublimation. Colors r vibrant and dries fast. Easy to install and is compatible with my epson peinter
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Reviewed in the United States on June 1, 2026
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Angela
Battle Creek, US
★★★★★ 5
Great quality, Great price
Color: 1BK/1C/1Y/1M
Excellent transfer and image quality. It really does work in an Epson Eco tank, as long as you never fill it with the Epson ink.
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Reviewed in the United States on April 25, 2026
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Sheena
Grantham, US
★★★★★ 5
Love
Color: 1BK/1C/1Y/1M
Works very well. Color is on point. Worth the money. No clogs. No leaks.
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Reviewed in the United States on May 6, 2026
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Momazon
Whiting, US
★★★★★ 5
Great overall
Color: 1BK/1C/1Y/1M
Great price, great quality and super easy to use!
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Reviewed in the United States on April 20, 2026
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Hazel
Pawtucket, US
★★★★★ 5
Easy to use vibrant colors
Color: 1BK/1C/1Y/1M
This is a great sublimation ink!! Easy to use, easy to fill my epson ecotank Great colors when heat pressing
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Reviewed in the United States on April 16, 2026

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