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Description
DCAF15/DDB1 His&StrepII Tag Protein, HumanProduct Specification Species Human Accession Q66K64Q16531 Amino Acid Sequence A2 L600 end (DCAF15), N His StrepII TEV GG M1 H1140 end (DDB1), No tag Expression System Baculovirus InsectCells Molecular Weight 69. 6 KDa 126. 9 KDa Conjugation Unconjugated Tag His Tag Physical Appearance Liquid Storage Buffer 50 mM Tris HCl (pH 7. 5), 200 mM NaCl, 20% glycerol, 1 mM DTT Stability & Storage 80 Guidelines Please use rapid thawing with running water to
Product Specification
| Species | Human |
| Accession | Q66K64、Q16531 |
| Amino Acid Sequence | A2-L600 end (DCAF15), N-His-StrepII-TEV-GG |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 69.6 KDa/126.9 KDa |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris-HCl (pH 7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT |
| Stability & Storage | -80℃ |
Guidelines
Please use rapid thawing with running water to thaw the protein samples.
For complete recovery, mix well and spin before use.
Product must not be stored in diluted solutions.
Aliquots below 10μl are not advisable.
Avoid repetitive freeze-thaw cycles!
For complete recovery, mix well and spin before use.
Product must not be stored in diluted solutions.
Aliquots below 10μl are not advisable.
Avoid repetitive freeze-thaw cycles!
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4.6 ★★★★★
Based on 26 reviews
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Product Reviews
★★★★★ 5
A wonderful primer for those discovering the amazing world of generative AI
Format: Paperback, Format: Paperback
Somewhere in the past year, ChatGPT has gone from "cool, interesting, amusing" to a massively valuable work assistant capable of writing Python scripts, analysing data, and doing lots, lots, more. The key to this? The rapid evolution of OpenAI's GPT models and making my first forays into prompt engineering.
If I thought I was getting good, though, this book took me reminded me that I'm just scratching the surface. Halfway through the first chapter I was already furiously scribbling notes in the margins for what I could do better with my prompt writing and by the end of the text I felt like I had gotten a very good grounding - not just in GPTs specifically but in the bigger picture of how these hugely powerful tools were trained and came to maturity.
I imagine that few will argue with my assertion that there is lots of hyperbole and "noise" in the AI space right now which, as ever, makes it hard to pick out the signal from the noise. Which is precisely why I sought out an O'Reilly title and I'm very glad that I did.
Thorough, excellent, and I hope that this edition will be the first of many. As this rapidly maturing field scales and matures I think that prompt engineering will be an essential discipline to master. Pick up this text to get a good foothold on things.
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Reviewed in the United States on August 3, 2024
★★★★★ 4
Understanding AI prompts as a science
Format: Audiobook
Prompt engineering is the art and science of finding the right words to generate the right responses from artificial intelligence (AI). It's becoming a skill in-demand in today's workplaces. It's also becoming an essential skill for life. To understand prompt engineering, you must understand how AI tools understand their inputs. This book explains that to you in many forms.
It covers prompting as it intersects all the major AI disciplines, like general prompts (as in a ChatBot), fine-tuning, and retrieval-augmented generation (RAG). It also introduces how prompting intersects a couple of image-generation tools.
As a scientist, I appreciate the theoretical approach to these practical matters. Too many people are hacks at AI these days, and any technical understanding can rapidly advance an individual's effectiveness. Prompt engineering is often made fun of as a "soft" topic, but as this book demonstrates, it intersects all the major AI areas. Presumably less garbage in means less garbage out. This book shows how you can make that take place.
As typical for O'Reilly materials, this book appeals to people who desire an intermediate-to-advanced understanding of how AI works. It's not for the casual user. Anyone technical involved in professional knowledge generation using AI can benefit from understanding the dynamics "under the hood." It's a good, though perhaps not ground-breaking, textbook for those of us unable to take a class in the subject. Prompt engineering may be a soft topic to many, but books like this ground the field in the science that can make or break a digital or software product.
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Reviewed in the United States on December 13, 2025
★★★★★ 5
Great book
Format: Paperback
Great book! It is helpful as I work towards my Masters of Science in Applied Artificial Intelligence
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Reviewed in the United States on December 20, 2025
★★★★★ 5
Comprehensive Guide with Practical Insights
Format: Paperback
This is a solid book for understanding the art and science of working with LLMs and other generative AI models. I always struggled with getting the output I was looking for, and wasn't sure how best to "ask" the models for what I wanted. This book did a great job of laying out the strategies and practical guidance to craft the prompts. There were a lot of tips and tricks, but the overall understanding and framework around prompt engineering has been super useful.
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Reviewed in the United States on June 25, 2024
★★★★★ 5
A must read!
Format: Paperback
Hands down, the best book on prompt engineering and implementing LLMs. Really enjoyed Michael and James deep dive. Whether you're technical or not, this book is foundational to a deeper understanding in how to properly explore and implement LLMs.
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Reviewed in the United States on June 25, 2024
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