SKU: 81588829602

Rat IFN-γ ELISA KIT

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Description

Rat IFN-γ ELISA KITProduct Specification Description Detection Principle: The double antibody sandwich ELISA method was used in this experiment. Anti rat IFN The monoclonal antibody was coated on the microplate, and IFN in samples and standards; It will bind to the antibody fixed on the plate, and the free components will be washed away; Biotin labeled anti rat IFN was added; Polyclonal antibody, and IFN bound to the microplate; Binding, free components are washed away;

Product Specification

Description

Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. Anti rat IFN-γThe monoclonal antibody was coated on the microplate, and IFN-γ in samples and standards; It will bind to the antibody fixed on the plate, and the free components will be washed away; Biotin labeled anti rat IFN-γ was added; Polyclonal antibody, and IFN-γ bound to the microplate; Binding, free components are washed away; The streptavidin labeled horseradish peroxidase, which specifically recognizes biotin, is added to form a complex. After washing away the free components, the substrate solution (chromogenic agent) is added, and the color of the solution gradually turns blue. After adding the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

Precoated 96 well plates, standards, anti rat IFN - γ One copy of detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions

Sensitivity: 15.6 pg/ml

Detection Range: 39.1 - 2500 pg/ml

Recovery Range: 89.0-118.9%

Storage Method: 2-8 ℃ span>

Standard Curve

Background:

IFN-γ (interferon -γ)It is a typical proinflammatory cytokine produced by a variety of immune cells under inflammatory conditions, especially by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen-presenting molecules, and immunoglobulin class switching in B cells. It also has antiviral, antiproliferative and apoptotic effects. In addition, IFN-γ Acts as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. Ifn-γ Dimers pass through two ifn-γ R1 and two ifn-γ The receptor complex of the R2 subunit signals.

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SKU: 81588829602

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4.1 ★★★★★
Based on 29 reviews
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M
Verified Purchase
Michelle Ruiz
Grantham, US
★★★★★ 4
Good toy for Kittens
Size: 30 Pcs
Seemed safe to play with your cat. Entertain to have them chase . Cute to seem cats hunt.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 29, 2025
S
Verified Purchase
Shelia I.
Houston, US
★★★★★ 5
Stormie approved.
Size: 30 Pcs
Stormie loves this!! Cute, fun, fair price
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Reviewed in the United States on February 11, 2026
B
Verified Purchase
Bobby
Dallas, US
★★★★★ 3
It's funny but cheap
My cat does enjoy chasing the little fluff balls around and then batting them for a few seconds than looking back at me for more. It's good for some good laughs and entertainment but the fluff balls and little plastic shooter are honestly dollar tree level quality. The shooter has one flimsy spring and looking at the placement inside it seems like something that'll fall apart pretty fast. Not worth $11 or $8, this should be >$5 easily.
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Reviewed in the United States on October 8, 2024
V
Verified Purchase
Victor
Louisville, US
★★★★★ 1
Dangerous
Size: 30 Pcs
My 1 year old cat loved to chase the balls, but who ever designed these balls should be ashamed of their selves. They balls are to small and easy for the cat to chew on and swallow!!! My cat just got done with an extremely costly surgery because he swallowed 3 of the balls he must of hid them somewhere because he usually did not play with them unattended. Never mind the pain my poor sweet baby just went through. I would never purchase something where somebody has not tested the size and shape of something to make sure that it is safe for an animal, sad to assume that somebody would create such a toy without checking that out and putting a animal through this nightmare. This is the first time in my that I have ever done a review, but I would never want somebody to go through what my cat and I have just experienced. Had to him to the emergency room on a Sunday to a follow up appointment at the vet on a Monday and then another follow up appointment on a Tuesday to redo the ultrasound to on Wednesday another appointment to check them and then him having to go in for an emergency surgery on Thursday. The problem is, he didn’t eat them all at one time so they obviously were sitting in his tummy until he started getting sick and throwing up. I will be checking into this company a bit deeper as well, so please be aware and don’t ever purchase something like this.
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Reviewed in the United States on September 19, 2024
M
Verified Purchase
Moment
Whiting, US
★★★★★ 5
All 3 cats say 5 stars!
Size: 100 Pcs
The cats love to chase these balls. And it comes with 100! Which is the perfect amount. So far the gun is still working well. I just find you need to barely put the pom pom in after cocking the gun. If you push the pom pom all the way in, it wont shoot. More often than not though, I just throw the balls for the cats. The gun shoots them very far, the cats enjoy chasing them, but they prefer to jump up and try to catch them, which they cant do when I use the gun
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Reviewed in the United States on January 26, 2025

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